The application of imaging flow cytometry to high-throughput biodosimetry
نویسندگان
چکیده
منابع مشابه
The Application of Imaging Flow Cytometry to High-Throughput Biodosimetry
Biodosimetry methods, including the dicentric chromosome assay, the cytokinesis-block micronucleus assay and the γH2AX marker of DNA damage are used to determine the dose of ionizing radiation. These techniques are particularly useful when physical dosimetry is absent or questioned. While these assays can be very sensitive and specific, the standard methods need to be adapted to increase sample...
متن کاملOptimized automated data analysis for the cytokinesis‐block micronucleus assay using imaging flow cytometry for high throughput radiation biodosimetry
The cytokinesis-block micronucleus (CBMN) assay is a well-established technique that can be employed in triage radiation biodosimetry to estimate whole body doses of radiation to potentially exposed individuals through quantitation of the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay has been partially automated using traditional microscope-based methods and mo...
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Molecular biodosimetry tools are a valuable asset for life-saving medical triage and patient management following a radiological or nuclear disaster. Even with the delayed onset of symptoms, sometimes several days after exposure, gene-expression biomarkers can identify these exposed individuals very early after exposure, allowing for prompt medical intervention. This early assessment of a radia...
متن کاملThe imaging flow cytometry cytokinesis‐block micronucleus assay for radiation biodosimetry
The cytokinesis-block micronucleus (CBMN) assay is an accepted method for biodosimetry where the dose of radiation to potentially exposed individuals is estimated from the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay is traditionally performed using a microscope-based scoring procedure which can be labour intensive, time consuming and subject to variability of...
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Flow cytometry (FCM) software packages from R/Bioconductor, such as flowCore and flowViz, serve as an open platform for development of new analysis tools and methods. We created plateCore, a new package that extends the functionality in these core packages to enable automated negative control-based gating and make the processing and analysis of plate-based data sets from high-throughput FCM scr...
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ژورنال
عنوان ژورنال: Genome Integrity
سال: 2017
ISSN: 2041-9414
DOI: 10.4103/2041-9414.198912